HBV GENOTYPING IN DIFFERENT CATEGORIES OF

HBV INFECTED PATIENTS

ABSTRACT

Aims: To highlight which genotype is the most prevalent cause of acute or chronic infection in HBV patients and also to enable us to find out the genotype in different subgroups of HBV patients.

Methods: Around 295 worked-up patients with HBV Serological markers positive acute and chronic liver disease, asymptomatic Carriers and patients with Cirrhosis and HCC were included in this study. PCR method was applied to study the prevalence of HBV genotype in Pakistan. The entire nucleotide sequence of human HBV genomes of various subtypes has been classified into six genotypes designated A-F.  Genotyping of HBV was performed by PCR of the surface gene of HBV. DNA was extracted from 200 ul of serum samples obtained HBV positive patients.

Results: Two hundred and ninety five HBsAg positive registered patients samples were received from the PMRC, 226(77%) were males, 69(23%) were females (M to F ratio 4:1). Out of 295 patients, 156(53.2%) had Acute (CAH), 71(24.2%) were HBV Carriers, 54(18.4%) had Chronic liver diseases (CLD). Cirrhosis and HCC patients were 14(4.7%). Genotype D were 208(70.5%) was the most prevalent genotype in all categories of HBV patients, Acute 108(69.2%), CLD 39(72.2%) and Carrier 53 (74.7%). Cirrhosis/HCC 8(57.1%) were. Genotype A were 60 (20.3%) was the second prevalent with 28 (18%) in acute cases, 12(22.2%) in CLD, 14(19.7%) in carriers and 6(42.9) Cirrhosis/HCC patients. Mixed genotype were 27 (9.1%) was found in 20(12.8%) of Acute patients, 3(5.6%) of CLD and 4(5.6%) of carriers.

Conclusion: Genotype D happens to be the most prevalent genotype in HBV patients followed by Genotype A and A+D. No significant difference was found in different subgroup of HBV patients.